Xavier Darzacq

Assistant Professor of Genetics, Genomics and Development Department of Molecular and Cell Biology
University of California, Berkeley

 

Our group’s general interest is to dissect how the assembly and 3D nuclear organization of the pre-initiation complex and chromatin can influence gene expression programs during differentiation. One of our group’s primary approaches to the study of transcriptional regulation has been to adopt and modify imaging hardware, algorithms and protocols for detecting single molecules in the nucleoplasm of living cells. These approaches are used to map and quantify protein and chromatin distribution in 3D with spatial resolutions of 15 to 30 nanometers, track how single proteins sample space to reach their targets, and count mRNAs at active transcription sites in order to assess transcriptional activity at the level of individual genes within single cells.